Fig. 1

Estrogen doesn’t modulate steady state mRNA message of MDR-1 but decreases protein level In this experiment, the control (vehicle only) and estrogen treated animals’ ovaries were removed and placed in TRizol for RNA extraction. RNA was then used to make complementary DNA. Quantitative Real-Time Polymerase Chain Reaction was performed using (ERβ) as a control that indicated the mice did actually receive the appropriate dosing. All samples were standardized to β-actin mRNA. The immunoblots were done by using whole ovary lysates of control and estrogen treated mice. Dosages of 1 μg and 10 μg were significantly decreased compared to controls. BCRP did not show a difference with our qPCR data, but we attempted to do immunoblotting without success due to unsuitable antibodies